Tuesday, December 20, 2011

Buffer for running agarose gel and preparing agarose gel?

There are several variations in the buffer depending on the purpose of the gel ( for example TBE (tris-borate- EDTA) versus TAE (Tris-acetate-EDTA) buffers) each used in gel separation of nucleic acids. TBE runs cooler but the DNA cannot generally be removed from the gel for continued use TAE runs hotter and thus must use a lower/slower voltage but the DNA can be removed and used further.

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